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The Guest Lecture on “CRISPR-based Genome Editing for Improvement in Agriculture and Healthcare” provided students with an insightful overview of one of the most transformative technologies in modern biotechnology and life sciences. The session introduced participants to the fundamental principles of CRISPR-Cas genome editing, including targeted gene modification, guide RNA, and the role of Cas enzymes in precise genetic manipulation. The lecture highlighted the potential of CRISPR technology in agriculture, particularly for developing improved crop varieties with enhanced productivity, nutritional value, stress tolerance, and resistance to diseases. Its applications in healthcare, including disease research, therapeutic development, diagnostics, and personalized medicine, were also discussed. The session helped students understand the opportunities as well as the ethical, safety, and regulatory considerations associated with genome editing. Through expert interaction and discussion, participants gained awareness of emerging research trends and interdisciplinary applications of genetic engineering. Overall, the lecture enhanced students’ scientific knowledge, research awareness, and understanding of the potential of CRISPR technology in addressing future agricultural and healthcare challenges.